Application of Cadherin cRNA Probes in Brains of Alzheimer’s Disease

Abstract

The cadherin superfamily molecules, functioning as cell adhesion molecules, are recognized to play roles in both physiological and pathological processes. The cadherin-based adherent junction (CAJ) is believed to interact with presenilin-1 (PS-1), suggesting that disruptions in CAJ structures might contribute to neurodegeneration, potentially leading to Alzheimer’s Disease (AD). Yet, the specific expression patterns of cadherin superfamily mRNA remain somewhat ambiguous. This research utilizes in situ Hybridization (ISH) to examine the expression and localization of cadherin mRNA in AD mouse model brains. Long cRNA probes targeting cadherin revealed endogenous mRNA expression in brain sections. Interestingly, senile plaques in the AD mouse brain are also bound to these probes. This binding, however, may not exclusively denote cadherin mRNA, as ISH detected both antisense and sense cRNA probes. Our data suggests that while antisense cRNA probes effectively detect cadherin mRNA expression in AD brain cells, their association with senile plaques might not specifically signify cadherin mRNA expression.