Application of Cadherin cRNA Probes in Brains of Alzheimer’s Disease
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Date
2024-03-17
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Publisher
Springer Nature
Abstract
The cadherin superfamily molecules, functioning as cell adhesion molecules, are recognized to
play roles in both physiological and pathological processes. The cadherin-based adherent
junction (CAJ) is believed to interact with presenilin-1 (PS-1), suggesting that disruptions in
CAJ structures might contribute to neurodegeneration, potentially leading to Alzheimer’s
Disease (AD). Yet, the specific expression patterns of cadherin superfamily mRNA remain
somewhat ambiguous. This research utilizes in situ Hybridization (ISH) to examine the
expression and localization of cadherin mRNA in AD mouse model brains. Long cRNA probes
targeting cadherin revealed endogenous mRNA expression in brain sections. Interestingly, senile
plaques in the AD mouse brain are also bound to these probes. This binding, however, may not
exclusively denote cadherin mRNA, as ISH detected both antisense and sense cRNA probes. Our
data suggests that while antisense cRNA probes effectively detect cadherin mRNA expression in
AD brain cells, their association with senile plaques might not specifically signify cadherin
mRNA expression.
Description
Journal article
Keywords
Alzheimer's disease, cadherin, in situ hybridization, cRNA probe, senile plaques
Citation
Zhou, H., Du, S.J., Gendi, F. et al. Application of Cadherin cRNA Probes in Brains of Alzheimer’s Disease Mouse Model. Mol Biol 58, 504–513 (2024). https://doi.org/10.1134/S0026893324700134