Physiological and molecular characterization of drought responses and identification of candidate tolerance genes in cassava

Simple item page
dc.contributor.authorTuryagyenda, Laban F.
dc.contributor.authorKizito, Elizabeth B.
dc.contributor.authorFerguson, Morag
dc.contributor.authorBaguma, Yona
dc.contributor.authorAgaba, Morris
dc.contributor.authorHarvey, Jagger J. W.
dc.contributor.authorOsiru, David S. O.
dc.date.accessioned2017-12-15T11:23:21Z
dc.date.accessioned2021-12-21T09:04:44Z
dc.date.available2017-12-15T11:23:21Z
dc.date.available2021-12-21T09:04:44Z
dc.date.issued2013-02
dc.descriptionThis study was carried out to identify candidate drought-tolerance genes and expression-based markers of drought stress in cassava. One drought-tolerant (improved variety) and one drought-susceptible (farmer-preferred) cassava landrace were grown in the glasshouse under well-watered and water-stressed conditions.en_US
dc.description.abstractCassava is an important root crop to resource-poor farmers in marginal areas, where its production faces drought stress constraints. Given the difficulties associated with cassava breeding, a molecular understanding of drought tolerance in cassava will help in the identification of markers for use in marker-assisted selection and genes for transgenic improvement of drought tolerance. This study was carried out to identify candidate drought-tolerance genes and expression-based markers of drought stress in cassava. One drought-tolerant (improved variety) and one drought-susceptible (farmer-preferred) cassava landrace were grown in the glasshouse under well-watered and water-stressed conditions. Their morphological, physiological and molecular responses to drought were characterized. Morphological and physiological measurements indicate that the tolerance of the improved variety is based on drought avoidance, through reduction of water loss via partial stomatal closure. Ten genes that have previously been biologically validated as conferring or being associated with drought tolerance in other plant species were confirmed as being drought responsive in cassava. Four genes (MeALDH, MeZFP, MeMSD and MeRD28) were identified as candidate cassava drought-tolerance genes, as they were exclusively up-regulated in the drought-tolerant genotype to comparable levels known to confer drought tolerance in other species. Based on these genes, we hypothesize that the basis of the tolerance at the cellular level is probably through mitigation of the oxidative burst and osmotic adjustment. This study provides an initial characterization of the molecular response of cassava to drought stress resembling field conditions. The drought-responsive genes can now be used as expression-based markers of drought stress tolerance in cassava, and the candidate tolerance genes tested in the context of breeding (as possible quantitative trait loci) and engineering drought tolerance in transgenics.en_US
dc.identifier.citationKizito EB, Turyagyenda LF, Ferguson M, Baguma Y, Agaba M, Harvey JJW, Osiru DSO. 2013. Physiological and molecular characterization of drought responses and identification of candidate tolerance genes in cassava. AoB PLANTS 5: plt007; doi:10.1093/aobpla/plt007en_US
dc.identifier.urihttps://hdl.handle.net/20.500.11951/83
dc.language.isoenen_US
dc.publisheraobplants.oxfordjournals.orgen_US
dc.subjectCassava drought avoidanceen_US
dc.subjectDrought tolerance-Cassavaen_US
dc.subjectGene expression-Cassavaen_US
dc.subjectOsmotic adjustment-Cassavaen_US
dc.subjectOxidative stress-Cassavaen_US
dc.titlePhysiological and molecular characterization of drought responses and identification of candidate tolerance genes in cassavaen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Kizito_Physiological and molecular characterization of drought responses and identification of candidate tolerance genes in cassava_2013.pdf
Size:
321.18 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Plain Text
Description:
Download
Collections
Faculty of Agricultural Sciences