Developing a yeast-based assay protocol to monitor total oestrogenic activity induced by 17β-oestradiol in activated sludge supernatants from batch experiments

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dc.contributor.authorWozei, Eleanor
dc.contributor.authorHermanowicz, SW
dc.date.accessioned2020-03-04T07:15:49Z
dc.date.accessioned2021-12-21T09:35:50Z
dc.date.available2020-03-04T07:15:49Z
dc.date.available2021-12-21T09:35:50Z
dc.date.issued2006-04-11
dc.descriptionThis is a research paper on developing a yeast-based assay protocol to monitor total oestrogenic activity induced by 17β-oestradiol in activated sludge supernatants from batch experimentsen_US
dc.description.abstractA yeast-based assay protocol developed for detecting oestrogenic activity in activated sludge (AS) supernatant is described. The protocol used Saccharomyces cerevisiae construct RMY/ER-ERE with human oestrogen receptor (ERα) and lacZ reporter genes, and was developed by modifying existing assays for use with AS samples from batch experiments. The method was able to detect total oestrogenic activity (without prior extraction) in supernatants of AS spiked with 17β-oestra¬diol (E2) with a detection limit of 0.03 ngE2-equivalent/ℓ and an overall quantification limit of 100 ngE2-equivalent/ℓ. Mean E2-induced oestrogenic activity recoveries of >56% were obtained from the spiked samples.en_US
dc.identifier.citationE Wozei and SW Hermanowicz. Developing a yeast-based assay protocol to monitor total oestrogenic activity induced by 17β-oestradiol in activated sludge supernatants from batch experiments. Lawrence Berkeley National Laboratory, Mail Stop 70A-3317, 1 Cyclotron Road, Berkeley, CA, 94720. 11th April, 2006. ISSN 0378-4738 = Water SA Vol. 32 No. 3 July 2006 ISSN 1816-7950 = Water SA (on-line)en_US
dc.identifier.urihttps://hdl.handle.net/20.500.11951/874
dc.language.isoenen_US
dc.publisherLawrence Berkeley National Laboratoryen_US
dc.subjectSludgeen_US
dc.subjectOestradiolen_US
dc.subjectOestrogenicen_US
dc.subjectSuspended solidsen_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.subjectYeasten_US
dc.titleDeveloping a yeast-based assay protocol to monitor total oestrogenic activity induced by 17β-oestradiol in activated sludge supernatants from batch experimentsen_US
dc.typeArticleen_US
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