Developing a yeast-based assay protocol to monitor total oestrogenic activity induced by 17β-oestradiol in activated sludge supernatants from batch experiments
Loading...
Date
2006-04-11
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Lawrence Berkeley National Laboratory
Abstract
A yeast-based assay protocol developed for detecting oestrogenic activity in activated sludge (AS) supernatant is described. The protocol used Saccharomyces cerevisiae construct RMY/ER-ERE with human oestrogen receptor (ERα) and lacZ reporter genes, and was developed by modifying existing assays for use with AS samples from batch experiments. The method was able to detect total oestrogenic activity (without prior extraction) in supernatants of AS spiked with 17β-oestra¬diol (E2) with a detection limit of 0.03 ngE2-equivalent/ℓ and an overall quantification limit of 100 ngE2-equivalent/ℓ. Mean E2-induced oestrogenic activity recoveries of >56% were obtained from the spiked samples.
Description
This is a research paper on developing a yeast-based assay protocol to monitor total oestrogenic activity induced by 17β-oestradiol in activated sludge supernatants from batch experiments
Keywords
Sludge, Oestradiol, Oestrogenic, Suspended solids, Saccharomyces cerevisiae, Yeast
Citation
E Wozei and SW Hermanowicz. Developing a yeast-based assay protocol to monitor total oestrogenic activity induced by 17β-oestradiol in activated sludge supernatants from batch experiments. Lawrence Berkeley National Laboratory, Mail Stop 70A-3317, 1 Cyclotron Road, Berkeley, CA, 94720. 11th April, 2006. ISSN 0378-4738 = Water SA Vol. 32 No. 3 July 2006 ISSN 1816-7950 = Water SA (on-line)