Christine Nabadda Mpaulo2025-11-192025-11-192025-11-10https://hdl.handle.net/20.500.11951/2055Postgraduate researchThis study examined the relationship between human resource (HR) practices, Perceived Organizational Support (POS), and job satisfaction at Mukono government hospital. Specifically, it explored the relationships between HR practices and job satisfaction, HR practices and POS, and POS and job satisfaction. Additionally, the study assessed the mediating effect of POS on the relationship between HR practices and job satisfaction. A descriptive case study design was adopted, with qualitative and quantitative research approaches. Data was collected from 81 respondents using questionnaires and 9 key informant interviews. Regression results show that HR practices (β = 0.399, p < .001) and POS (β = 0.637, p < 0.001) positively influence job satisfaction and jointly account for 58.6% of the variations in job satisfaction (Adj R 2 = 0.586). HR practices were further found to positively influence POS (β = 0.232, p < .05). POS was also found to mediate the relationship between HR practices and job satisfaction (β = 0.358, p > .001). Based on these findings, the study concluded that HR practices are critical in enhancing job satisfaction. POS independently improves employee satisfaction and mediates the relationship between HR practices and job satisfaction. It is recommended that the hospital management should enhance HR practices by providing better working conditions, offering fringe benefits, and creating growth opportunities to improve job satisfaction. Additionally, HR practitioners should foster a supportive work environment that values employee well- being to strengthen perceptions of organizational support and further enhance satisfaction levels among staff.enThesis