Genetic and Root Growth Studies in Cassava (Manihot esculenta Crantz): Implications for Breeding
Kizito, Elizabeth B.
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Cassava (Manihot esculenta Crantz) is a perennial tropical crop grown for its starchcontaining tuberous roots. It is cultivated mainly by small-scale farmers and consumed daily by an estimated 500 million people. Cassava mosaic disease (CMD) has long been recognized as a major limiting factor to cassava production in Africa and severe epidemics hit Uganda in the late 1920’s and late 1980’s. In spite of its importance as a major food crop it is the least researched major crop and many questions regarding its genetics are still unresolved. This thesis has therefore dealt with studies on: i) the effect of CMD on the genetic diversity of cassava in Uganda, ii) the composition of varieties and the genetic structure within and between varieties on small farms in Uganda, iii) the genetic basis of two agronomic important traits, cyanogenic glucoside potential (CNP) and dry matter content (DMC) in cassava roots, using quantitative trait loci (QTL) mapping and iv) the effect of nutrient availability on the growth and tuber formation. The outcome of these studies is relevant for developing strategies for breeding and gene conservation programmes. CMD did not have a strong selective effect on the genetic diversity of cassava in Uganda in spite of earlier reports on losses of varieties due to the latest CMD epidemic. However, a loss of rare alleles in areas with high CMD incidence in Uganda was found. The composition of varieties differed widely between villages and districts and the genetic variation was surprisingly large within varieties although the variation was larger among varieties. The like-named varieties in different villages were genetically similar, demonstrating farmers’ ability to differentiate and maintain the same variety over large areas. We detected two QTL on two different linkage groups controlling CNP and six QTL on four different linkage groups controlling DMC. One QTL for CNP and one QTL for DMC mapped near each other, suggesting pleiotrophy or linkage of QTL. In the root studies, production of storage roots was found to be regulated by nutrient availability and appeared to be positively affected by a gradually increasing limitation of mineral nutrients during the growth of the plants.
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Kizito, Elizabeth B.; Bua, Anton; Fregene, Martin; Egwang, Thomas; Gullberg, Urban; Westerbergh, Anna (Springer International Publishing, 2005)Cassava (Manihot esculenta) is a tropical crop that is grown in Africa, Latin America and Southeast Asia. Cassava was introduced from Latin America into West and East Africa at two independent events. In Uganda a serious ...
Kizito, Elizabeth B.; Turyagyenda, L.F.; Ferguson, M.E.; Baguma, Y.; Harvey, J.W.; Gibson, P.; Wanjala, B.W.; Osiru, D.S.O. (African Crop Science Society Journal, 2012)Understanding of genetic diversity among a breeding population is an important requirement for crop improvement as it allows for the selection of diverse parental combinations and formation of heterotic pools for genetic ...
Physiological and molecular characterization of drought responses and identification of candidate tolerance genes in cassava Turyagyenda, Laban F.; Kizito, Elizabeth B.; Ferguson, Morag; Baguma, Yona; Agaba, Morris; Harvey, Jagger J. W.; Osiru, David S. O. (aobplants.oxfordjournals.org, 2013-02)Cassava is an important root crop to resource-poor farmers in marginal areas, where its production faces drought stress constraints. Given the difficulties associated with cassava breeding, a molecular understanding of ...